Molecules | |
Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides | |
Shigetaka Nakamura1  Shinzi Ogasawara1  Shigeo Matuda1  Isao Saito1  | |
[1] 1School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan 2Department of Synthetic Chemistry and Biological Chemistry, Factly of Enginnering, Kyoto University, Kyoto 606-8501, Japan 3Research Center for Bio-Architecture, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan | |
关键词: photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology; | |
DOI : 10.3390/molecules17010163 | |
来源: mdpi | |
【 摘 要 】
We demonstrated that 5-vinyldeoxyuridine (VU) and 5-carboxyvinyldeoxyuridine (CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any side reactions. Moreover, by performing irradiation at 312 nm, these photoligated ODNs were reversed to the original ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm for 15 min. Finally, we made a self-assembled structure with an ODN containing CVU and observed the photoligated ODN by photoirradiation.
【 授权许可】
CC BY
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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