Production and Radioprotective Effects of Pyrroloquinoline Quinone

International Journal of Molecular Sciences

Xiang-Hua Xiong³  Yan Zhao⁴  Xin Ge³  Shou-Jun Yuan²  Jian-Hua Wang³  Jing-Juan Zhi¹  Yan-Xin Yang¹  Bao-Hua Du¹  Wan-Jun Guo²  Shan-Shan Wang²  De-Xuan Yang² 

[1] School of Life Science and Biochemical Pharmaceutical, Shenyang Pharmaceutical University, Shenyang 110016, China; E-Mails:;Department of Pharmacology, Beijing Institute of Radiation Medicine, Beijing 100850, China; E-Mails:;Laboratory of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China; E-Mails:;Central Laboratory, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China; E-Mail:

关键词: methylotroph;    pyrroloquinoline quinine;    radioprotective;    hematopoiesis;    survival;   

DOI  :  10.3390/ijms12128913

来源: mdpi

【 摘 要 】

Pyrroloquinoline quinone (PQQ) was produced by fermentation of the Methylovorus sp. MP688 strain and purified by ion-exchange chromatography, crystallization and recrystallization. The yield of PQQ reached approximately 125 mg/L and highly pure PQQ was obtained. To determine the optimum dose of PQQ for radioprotection, three doses (2 mg/kg, 4 mg/kg, 8 mg/kg) of PQQ were orally administrated to the experimental animals subjected to a lethal dose of 8.0 Gy in survival test. Survival of mice in the irradiation + PQQ (4 mg/kg) group was found to be significantly higher in comparison with the irradiation and irradiation + nilestriol (10 mg/kg) groups. The numbers of hematocytes and bone marrow cells were measured for 21 days after sublethal 4 Gy gamma-ray irradiation with per os of 4 mg/kg of PQQ. The recovery of white blood cells, reticulocytes and bone marrow cells in the irradiation + PQQ group was faster than that in the irradiation group. Furthermore, the recovery of bone marrow cell in the irradiation + PQQ group was superior to that in irradiation + nilestriol group. Our results clearly indicate favourable effects on survival under higher lethal radiation doses and the ability of pyrroloquinoline quinine to enhance haemopoietic recovery after sublethal radiation exposure.

【 授权许可】

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.