Global Chemical Composition and Antioxidant and Anti-Tuberculosis Activities of Various Extracts of Globularia alypum L. (Globulariaceae) Leaves

Molecules

Daycem Khlifi¹  Moktar Hamdi¹  Akrem El Hayouni¹  Sylvie Cazaux¹  Jean Pierre Souchard¹  François Couderc¹ 

[1]¹Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique UMR CNRS 5623, University of Toulouse, University of Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, France

关键词: Globularia alypum L.;    polyphenols;    antioxidant activity;    anti-tuberculosis activity;   

DOI  :  10.3390/molecules161210592

来源: mdpi

【 摘 要 】

In this work, an evaluation of the biological activities of Globularia alypum L. extracts and their global chemical composition was realized. Extracts from G. alypum were obtained by two extraction methods. The composition of polyphenols (8.5–139.95 g gallic acid equivalent/Kg of dry mass), tannins (1.39–18.65 g catechin equivalent/Kg of dry mass), anthocyanins (8.17–70.69 mg cyanidin equivalent/Kg of dry mass) and flavonoids (0.31–19.28 g quercetin equivalent/Kg of dry mass) was evaluated. The samples were subjected to a screening for their antioxidant activities using the DPPH^· and ABTS^·+ assays. For the first time, the anti-tuberculosis activity (H₃₇Rv) for G. alypum was tested against Mycobacterium tuberculosis. The strongest antioxidant activity was obtained for the methanol extract (IC₅₀ = 15.58 ± 0.168 mg/L) and the best anti-tuberculosis activity was obtained for the petroleum ether extract (IC₅₀ = 77 mg/L). We have found a positive correlation between the total phenolics content and the antioxidant activity R² = 0.88 (DPPH^·) and R² = 0.97 (ABTS^·+). We have found also a positive correlation between the flavonoid content and the antioxidant activity R² = 0.91 (DPPH^·) and R² = 0.91 (ABTS^·+).

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.