Combined Extraction Processes of Lipid from Chlorella vulgaris Microalgae: Microwave Prior to Supercritical Carbon Dioxide Extraction

International Journal of Molecular Sciences

Céline Dejoye²  Maryline Abert Vian²  Guy Lumia¹  Christian Bouscarle¹  Frederic Charton¹ 

[1] CEA DEN Marcoule, Laboratory of Supercritical fluids and membranes, F-30207 Bagnols sur Cèze, France; E-Mails:;University of Avignon and Countries of Vaucluse, INRA, UMR408, F-84000 Avignon, France; E-Mails:

关键词: microalgae;    supercritical carbon dioxide;    microwave;    GC;    MS;    lipid extraction;   

DOI  :  10.3390/ijms12129332

来源: mdpi

【 摘 要 】

Extraction yields and fatty acid profiles from freeze-dried Chlorella vulgaris by microwave pretreatment followed by supercritical carbon dioxide (MW-SCCO₂) extraction were compared with those obtained by supercritical carbon dioxide extraction alone (SCCO₂). Work performed with pressure range of 20–28 Mpa and temperature interval of 40–70 °C, gave the highest extraction yield (w/w dry weight) at 28 MPa/40 °C. MW-SCCO₂ allowed to obtain the highest extraction yield (4.73%) compared to SCCO₂ extraction alone (1.81%). Qualitative and quantitative analyses of microalgae oil showed that palmitic, oleic, linoleic and α-linolenic acid were the most abundant identified fatty acids. Oils obtained by MW-SCCO₂ extraction had the highest concentrations of fatty acids compared to SCCO₂ extraction without pretreatment. Native form, and microwave pretreated and untreated microalgae were observed by scanning electronic microscopy (SEM). SEM micrographs of pretreated microalgae present tearing wall agglomerates. After SCCO₂, microwave pretreated microalgae presented several micro cracks; while native form microalgae wall was slightly damaged.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.