Mass Spectrometry Based Lipidomics: An Overview of Technological Platforms

Metabolites

Harald C. Kler²  Alexander Fauland¹  Gerald N. Rechberger³ 

[1]Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010 Graz, Austria

[2]Core Facility for Mass Spectrometry, Medical University of Graz, Stiftingtalstrasse 24, 8010 Graz, Austria

[3]Department for Molecular Biosciences, University of Graz, Humboldtstrasse 50/II, 8010 Graz, Austria

关键词: lipidomics;    mass spectrometry;    LC-MS;    shotgun;    ESI;    MALDI-TOF;   

DOI  :  10.3390/metabo2010019

来源: mdpi

【 摘 要 】

One decade after the genomic and the proteomic life science revolution, new ‘omics’ fields are emerging. The metabolome encompasses the entity of small molecules—Most often end products of a catalytic process regulated by genes and proteins—with the lipidome being its fat soluble subdivision. Within recent years, lipids are more and more regarded not only as energy storage compounds but also as interactive players in various cellular regulation cycles and thus attain rising interest in the bio-medical community. The field of lipidomics is, on one hand, fuelled by analytical technology advances, particularly mass spectrometry and chromatography, but on the other hand new biological questions also drive analytical technology developments. Compared to fairly standardized genomic or proteomic high-throughput protocols, the high degree of molecular heterogeneity adds a special analytical challenge to lipidomic analysis. In this review, we will take a closer look at various mass spectrometric platforms for lipidomic analysis. We will focus on the advantages and limitations of various experimental setups like ‘shotgun lipidomics’, liquid chromatography—Mass spectrometry (LC-MS) and matrix assisted laser desorption ionization-time of flight (MALDI-TOF) based approaches. We will also examine available software packages for data analysis, which nowadays is in fact the rate limiting step for most ‘omics’ workflows.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.