Comparative Chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)

Metabolites

Christian Rank²  Marie Louise Klejnstrup²  Lene Maj Petersen²  Sara Kildgaard²  Jens Christian Frisvad²  Charlotte Held Gotfredsen¹ 

[1] Department of Chemistry, Technical University of Denmark, Kemitorvet B201, DK-2800 Kgs. Lyngby, Denmark;Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark;

关键词: Aspergillus oryzae;    (RIB40);    Aspergillus flavus;    (NRRL 3357);    parasiticolide;    ditryptoleucine;    oryzamide;   

DOI  :  10.3390/metabo2010039

来源: mdpi

【 摘 要 】

Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.