Development of Novel Microsatellite Markers in the Omei Treefrog (Rhacophorus omeimontis)

International Journal of Molecular Sciences

Mian Zhao¹  Ruiping Zhang¹  Chenliang Li¹  Taiyang Mu¹  Shichao Wei¹  Xiong Li¹ 

[1] Animal Behaviour Research Group, College of Life Sciences, Central China Normal University, 152 Luoyulu, Hongshan District, Wuhan 430079, China;

关键词: Omei treefrog (Rhacophorus omeimontis);    microsatellite markers;    heterozygosity;    mate choice;   

DOI  :  10.3390/ijms13010552

来源: mdpi

【 摘 要 】

Eleven novel microsatellite markers were developed and characterized for the Omei treefrog (Rhacophorus omeimontis) using the fast isolation by AFLP of sequences containing repeats method. Polymorphism of each locus was tested in 24 individuals from two wild populations. The number of alleles per locus ranged from 4 to 15, the average observed and expected heterozygosity per locus ranged from 0.250 to 0.839 and from 0.562 to 0.914, respectively. Two of the 11 microsatellite loci showed significant deviations from Hardy-Weinberg equilibrium. Two locus pairs showed significant linkage disequilibrium. Neither evidence of scoring error due to stuttering nor evidence of large allele dropout was found at all of the 11 loci, but evidence of null alleles was indicated at two loci because of general excess of homozygotes for most allele size classes. These polymorphic loci will be useful markers in studying mate choice of the Omei treefrog.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.