Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

Biosensors

Emma B. Setterington¹ 

[1] Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA; E-Mail

关键词: electrochemical biosensor;    pathogen detection;    magnetic polyaniline;    screen-printed carbon electrode;    cyclic voltammetry;   

DOI  :  10.3390/bios2010015

来源: mdpi

【 摘 要 】

Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pure culture. Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results. An immunofunctionalized magnetic/polyaniline core/shell nano-particle (c/sNP) is employed to extract target cells from the sample solution and magnetically position them on a screen-printed carbon electrode (SPCE) sensor. The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE. This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.