Synthesis of Hyperbranched Glycoconjugates by the Combined Action of Potato Phosphorylase and Glycogen Branching Enzyme from Deinococcus geothermalis

Polymers

Jeroen van der Vlist¹  Martin Faber¹  Lizette Loen¹  Teunis J. Dijkman¹  Lia A.T.W. Asri¹ 

[1] Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands;

关键词: glycoconjugates;    biohybrids;    glycosyltransferases;    branching enzyme;    potato phosphorylase;    hyperbranched;    enzymatic polymerization;   

DOI  :  10.3390/polym4010674

来源: mdpi

【 摘 要 】

Potato phosphorylase is able to synthesize linear polyglucans from maltoheptaose primers. By coupling maltoheptaose to butane diamine, tris(2-aminoethyl)amine and amine functionalized amine functionalized poly ethyleneglycol (PEG), new primer molecules became available. The resulting di-, tri- and macro-primers were incubated with potato phosphorylase and glycogen branching enzyme from Deinococcus geothermalis. Due to the action of both enzymes, hyperbranched polyglucan arms were grown from the maltoheptaose derivatives with a maximum degree of branching of 11%. The size of the synthesized hyperbranched polyglucans could be controlled by the ratio monomer over primer. About 60%–80% of the monomers were incorporated in the glycoconjugates. The resulting hyperbranched glycoconjugates were subjected to Dynamic Light Scattering (DLS) measurements in order to determine the hydrodynamic radius and it became obvious that the structures formed agglomerates in the range of 14–32 nm.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.