Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells

Molecules

Keivan Zandi¹  Rafidah Lani¹  Pooi-Fong Wong¹  Boon-Teong Teoh¹  Sing-Sin Sam¹  Jefree Johari¹  Mohd Rais Mustafa¹ 

[1] ¹Tropical Infectious Diseases Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia ²Department of Pharmacology, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia

关键词: antiviral;    dengue;    flavones;    flavonoid;    molecule;   

DOI  :  10.3390/molecules17032437

来源: mdpi

【 摘 要 】

This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 μg/mL) significantly increased the number of DENV-2 foci by 35.66% ± 1.52 and 49.66% ± 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33% ± 2.11 and 89% ± 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 μg/mL) did not promote or inhibit Vero cell proliferation. The CC₅₀ value of flavone against Vero cells was 446 µg/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.