Comparison of Culture and Molecular Identification of Bacteria in Chronic Wounds

International Journal of Molecular Sciences

Daniel D. Rhoads¹  Randall D. Wolcott¹  Yan Sun² 

[1] Southwest Regional Wound Care Center, Lubbock, TX 79410, USA;Research and Testing Laboratory, Lubbock, TX 79407, USA

关键词: wound;    diagnosis;    biofilm;    molecular;   

DOI  :  10.3390/ijms13032535

来源: mdpi

【 摘 要 】

Clinical diagnostics of chronic polymicrobial infections, such as those found in chronic wounds, represent a diagnostic challenge for both culture and molecular methods. In the current retrospective study, the results of aerobic bacterial cultures and culture-free bacterial identification using DNA analyses were compared. A total of 168 chronic wounds were studied. The majority of bacteria identified with culture testing were also identified with molecular testing, but the majority of bacteria identified with the molecular testing were not identified with culture testing. Seventeen (17) different bacterial taxa were identified with culture, and 338 different bacterial taxa were identified with molecular testing. This study demonstrates the increased sensitivity that molecular microbial identification can have over culture methodologies, and previous studies suggest that molecular bacterial identification can improve the clinical outcomes of patients with chronic wounds.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.