Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis

Sensors

Chao-Wang Young¹  Jia-Ling Hsieh¹ 

[1] Department of Biomechatronic Engineering, National Chiayi University, No. 300, University Rd., East Dist., Chiayi 600, Taiwan;

关键词: electroosmotic flow;    cell lysis;    dielectrophoresis force;    cell manipulation;   

DOI  :  10.3390/s120302400

来源: mdpi

【 摘 要 】

This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland