Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman

International Journal of Molecular Sciences

Jaciana S. Aguiar³  Rosilma O. Araújo³  Maria do Desterro Rodrigues³  Kêsia X. F. R. Sena³  André M. Batista¹  Maria M. P. Guerra¹  Steno L. Oliveira²  Josean F. Tavares²  Marcelo S. Silva²  Silene C. Nascimento³ 

[1] Andrology Laboratory, Veterinary Medicine Department, Rural Federal University of Pernambuco, 52171-900, Recife, PE, Brasil; E-Mails:;Laboratory of Pharmaceutical Technology, Department of Pharmaceutical Sciences, Federal University of Paraiba, Cx. Postal 5009, 58051-970, João Pessoa, Paraíba, Brasil; E-Mails:;Antibiotics of Departament, Federal University of Pernambuco, 50670-901, Recife, PE, Brasil; E-Mails:

关键词: Erythroxylum caatingae;    antimicrobial activity;    cytotoxic activity;    hemolytic activity;    apoptosis;   

DOI  :  10.3390/ijms13044124

来源: mdpi

【 摘 要 】

In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6β-Benzoyloxy-3α-(3,4,5- trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 μg/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95:5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95:5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.