Silencing Quorum Sensing through Extracts of Melicope lunu-ankenda

Sensors

Li Ying Tan¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;

关键词: anti-quorum sensing;    bioluminescence;    lecA::lux;    Malaysian plants;    N-acyl-l-homoserine lactones (AHL);    Pseudomonas aeruginosa PAO1;    pyocyanin;    virulence;    swarming motility;   

DOI  :  10.3390/s120404339

来源: mdpi

【 摘 要 】

Quorum sensing regulates bacterial virulence determinants, therefore making it an interesting target to attenuate pathogens. In this work, we screened edible, endemic plants in Malaysia for anti-quorum sensing properties. Extracts from Melicope lunu-ankenda (Gaertn.) T. G. Hartley, a Malay garden salad, inhibited response of Chromobacterium violaceum CV026 to N-hexanoylhomoserine lactone, thus interfering with violacein production; reduced bioluminescence expression of E. coli [pSB401], disrupted pyocyanin synthesis, swarming motility and expression of lecA::lux of Pseudomonas aeruginosa PAO1. Although the chemical nature of the anti-QS compounds from M. lunu-ankenda is currently unknown, this study proves that endemic Malaysian plants could serve as leads in the search for anti-quorum sensing compounds.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.