Screening of New Microsatellite DNA Markers from the Genome of Platyeriocheir formosa

International Journal of Molecular Sciences

Hui-Ling Cheng²  Yan-Horn Lee³  Dai-Shion Hsiung¹ 

[1] Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan; E-Mail:;Department of Environmental Resources Management, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan; E-Mail:;Biotechnology Division, Fisheries Research Institute Council of Agriculture, Tungkang, Pingtung 928, Taiwan; E-Mail:

关键词: catadromous;    crab;    cross-specific amplification;    polymorphic loci;   

DOI  :  10.3390/ijms13055598

来源: mdpi

【 摘 要 】

The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To conserve P. formosa population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote the study of its population genetics in the future. In this study, more than 70 microsatellite sequences were found. Among these, 18 loci were selected to analyze the genetic diversity of P. formosa. With the exception of the Pfo15 locus, all of the remaining loci were polymorphic with allelic numbers ranging from 3–14. Heterozygosity within all 17 polymorphic loci ranged from 0.2–0.95 with an average of 0.55, which suggested that these loci are proper markers for studying population genetics. After we tested cross-specific amplification, eight and six primer sets could be successfully used for the amplification of microsatellite loci in morphologically similar Eriocheir sinensis and E. japonica, respectively; this suggests that they are useful markers for closely related species.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.