Astaxanthin Treatment Reduced Oxidative Induced Pro-Inflammatory Cytokines Secretion in U937: SHP-1 as a Novel Biological Target

Marine Drugs

Lorenza Speranza¹  Mirko Pesce¹  Antonia Patruno²  Sara Franceschelli²  Maria Anna de Lutiis²  Alfredo Grilli² 

[1] Department of Medicine and Science of Aging, University G. D’Annunzio-Chieti, Chieti 66100, Italy;

关键词: carotenoids;    SHP-1 protein;    inflammation;    astaxanthin;   

DOI  :  10.3390/md10040890

来源: mdpi

【 摘 要 】

It has been suggested that oxidative stress activates various intracellular signaling pathways leading to secretion of a variety of pro-inflammatory cytokines and chemokines. SHP-1 is a protein tyrosine phosphatase (PTP) which acts as a negative regulator of immune cytokine signaling. However, intracellular hydrogen peroxide (H₂O₂), generated endogenously upon stimulation and exogenously from environmental oxidants, has been known to be involved in the process of intracellular signaling through inhibiting various PTPs, including SHP-1. In this study, we investigated the potential role of astaxanthin, an antioxidant marine carotenoid, in re-establishing SHP-1 negative regulation on pro-inflammatory cytokines secretion in U-937 cell line stimulated with oxidative stimulus. ELISA measurement suggested that ASTA treatment (10 µM) reduced pro-inflammatory cytokines secretion (IL-1β, IL-6 and TNF-α) induced through H₂O₂, (100 µM). Furthermore, this property is elicited by restoration of basal SHP-1 protein expression level and reduced NF-κB (p65) nuclear expression, as showed by western blotting experiments.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.