Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates

Molecules

Antonio Cardoso¹  Clarissa Izabel M. Lins¹  Ednaldo Ramos dos Santos¹  Marta C. Freitas Silva¹ 

[1] ¹Northeastern Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco, 50050-900 Recife, PE, Brazil

关键词: Rhizopus arrhizus;    chitosan;    biopolymer;    agroindustrial substrates;   

DOI  :  10.3390/molecules17054904

来源: mdpi

【 摘 要 】

This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (I_C), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.