A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection

Sensors

Onursal Onen¹  Alper Sisman¹  Nathan D. Gallant¹  Patricia Kruk² 

[1] Department of Mechanical Engineering, University of South Florida, 4202 E. Fowler Avenue, ENB 118, Tampa, FL 33620, USA; E-Mails:;Department of Pathology and Cell Biology, University of South Florida Health, 12901 Bruce B. Downs Blvd., MDC 11, Tampa, FL 33612, USA; E-Mail:

关键词: ovarian cancer;    MEMS;    piezoelectric;    sensor;    early detection;    Bcl-2;    SH;    SAW;    SAM;    ultrasound;   

DOI  :  10.3390/s120607423

来源: mdpi

【 摘 要 】

In this study, the design, fabrication, surface functionalization and experimental characterization of an ultrasonic MEMS biosensor for urinary anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) detection with sub ng/mL sensitivity is presented. It was previously shown that urinary Bcl-2 levels are reliably elevated during early and late stages of ovarian cancer. Our biosensor uses shear horizontal (SH) surface acoustic waves (SAWs) on surface functionalized ST-cut Quartz to quantify the mass loading change by protein adhesion to the delay path. SH-SAWs were generated and received by a pair of micro-fabricated interdigital transducers (IDTs) separated by a judiciously designed delay path. The delay path was surface-functionalized with monoclonal antibodies, ODMS, Protein A/G and Pluronic F127 for optimal Bcl-2 capture with minimal non-specific adsorption. Bcl-2 concentrations were quantified by the resulting resonance frequency shift detected by a custom designed resonator circuit. The target sensitivity for diagnosis and identifying the stage of ovarian cancer was successfully achieved with demonstrated Bcl-2 detection capability of 500 pg/mL. It was also shown that resonance frequency shift increases linearly with increasing Bcl-2 concentration.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.