Nutrients | |
Neonatal Phosphate Nutrition Alters |
|
Lindsey S. Alexander1  Brynn S. Seabolt1  Robert P. Rhoads2  | |
[1] Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA;Department of Animal & Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; | |
关键词: satellite cell; phosphate; neonatal; pig; | |
DOI : 10.3390/nu4060436 | |
来源: mdpi | |
【 摘 要 】
Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4) deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW) were pair-fed liquid diets that were either PO4 adequate (0.9% total P), supra-adequate (1.2% total P) in PO4 requirement or deficient (0.7% total P) in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190044218ZK.pdf | 218KB | download |