Biosensors | |
Peroxide-Dependent Analyte Conversion by the Heme Prosthetic Group, the Heme Peptide “Microperoxidase-11” and Cytochrome c on Chitosan Capped Gold Nanoparticles Modified Electrodes | |
Aysu Yarman1  Bettina Neumann1  Maria Bosserdt1  Nenad Gajovic-Eichelmann1  | |
[1] Fraunhofer Institute for Biomedical Engineering, IBMT, D-14476 Potsdam, Germany; | |
关键词: peroxide dependent catalysis; hemin; microperoxidase-11; cytochrome c; | |
DOI : 10.3390/bios2020189 | |
来源: mdpi | |
【 摘 要 】
In view of the role ascribed to the peroxidatic activity of degradation products of cytochrome c (cyt c) in the processes of apoptosis, we investigate the catalytic potential of heme and of the cyt c derived heme peptide MP-11 to catalyse the cathodic reduction of hydrogen peroxide and to oxidize aromatic compounds. In order to check whether cyt c has an enzymatic activity in the native state where the protein matrix should suppress the inherent peroxidatic activity of its heme prosthetic group, we applied a biocompatible immobilization matrix and very low concentrations of the co-substrate H2O2. The biocatalysts were entrapped on the surface of a glassy carbon electrode in a
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190044082ZK.pdf | 396KB | download |