Molecules | |
Physicochemical Characterization and Cytotoxic Activity Evaluation of Hydroxymethylferrocene:β-Cyclodextrin Inclusion Complex | |
Rosa Iacovino1  Jolanda Valentina Caso1  Filomena Rapuano1  Agostino Russo1  Marina Isidori1  Margherita Lavorgna1  Gaetano Malgieri1  | |
[1] 1Department of Environmental Science, Second University of Naples, via A.Vivaldi 43, 81100 Caserta, Italy 2Department of Biological and Environmental Science, University of Sannio, via Port'Arsa, 11, 82100 Benevento, Italy 3Department of Life Sciences, Second University of Naples, via A.Vivaldi 43, 81100 Caserta, Italy | |
关键词: β-cyclodextrin; hydroxymethylferrocene; inclusion complex; stability constant; phase solubility diagram; cytotoxicity; | |
DOI : 10.3390/molecules17056056 | |
来源: mdpi | |
【 摘 要 】
An inclusion complex of hydroxymethylferrocene (FeMeOH) with β-cyclodextrin (β-CD) was prepared in the solid state by different techniques such as physical mixture, coprecipitation, kneading and freeze-drying. The formation of the inclusion complex was confirmed by X-ray Powder Diffractometry and Fourier Transform-Infrared spectroscopy. In aqueous solution, the 1:1 stoichiometry was established by a Job plot. The inclusion complex formation was also investigated by NMR and the stability constant (Kb) of the complex was determined to be 478 M−1, which is in agreement with that obtained with UV-Vis tritation (Kb = 541.3 M−1). The phase solubility study showed a diagram classified as BS type and that the solubility of FeMeOH was slightly increased in the presence of β-CD. Furthermore, utilizing phase solubility diagram data, the Kb was estimated to be equal to 528.0 M−1. The cytotoxic activity of FeMeOH and its complexation product with β-CD was determined using the MTT-assay on MDA-MB-231 cell line, showing that the inclusion complex has a higher capability of inhibiting cell growth compared to that of pure FeMeOH.
【 授权许可】
CC BY
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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