Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells

Sensors

Chun-Ping Jen¹  Tamara G. Amstislavskaya²  Ya-Hui Liu¹  Ju-Hsiu Hsiao¹ 

[1] Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovation, National Chung Cheng University, Chia Yi, 62102, Taiwan; E-Mails:;Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia; E-Mail:

关键词: microwell;    single-cell;    electric lysis;    electroosmotic-driven;    microfluidics;   

DOI  :  10.3390/s120606967

来源: mdpi

【 摘 要 】

Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-μm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland