Plants versus Fungi and Oomycetes: Pathogenesis, Defense and Counter-Defense in the Proteomics Era

International Journal of Molecular Sciences

Abdelbasset El Hadrami¹  Ahmed F. El-Bebany¹  Zhen Yao¹  Lorne R. Adam¹  Ismailx El Hadrami² 

[1] Department of Plant Science, University of Manitoba, 222, Agriculture Building, Winnipeg, Manitoba, R3T 2N2, Canada; E-Mails:;Laboratoire de Biotechnologies, Protection et Valorisation des Ressources Végétales (Biotec-VRV), Faculté des Sciences Semlalia, B.P. 2390, Marrakech, 40 000, Morocco; E-Mail:

关键词: biotrophs;    counter-defenses;    defenses;    effectors;    hemibiotrophs;    necrotrophs;    pathogenicity factors;    plant-pathogen interactions;    pathosystems;    proteome;    proteomics approach;   

DOI  :  10.3390/ijms13067237

来源: mdpi

【 摘 要 】

Plant-fungi and plant-oomycete interactions have been studied at the proteomic level for many decades. However, it is only in the last few years, with the development of new approaches, combined with bioinformatics data mining tools, gel staining, and analytical instruments, such as 2D-PAGE/nanoflow-LC-MS/MS, that proteomic approaches thrived. They allow screening and analysis, at the sub-cellular level, of peptides and proteins resulting from plants, pathogens, and their interactions. They also highlight post-translational modifications to proteins, e.g., glycosylation, phosphorylation or cleavage. However, many challenges are encountered during in planta studies aimed at stressing details of host defenses and fungal and oomycete pathogenicity determinants during interactions. Dissecting the mechanisms of such host-pathogen systems, including pathogen counter-defenses, will ensure a step ahead towards understanding current outcomes of interactions from a co-evolutionary point of view, and eventually move a step forward in building more durable strategies for management of diseases caused by fungi and oomycetes. Unraveling intricacies of more complex proteomic interactions that involve additional microbes, i.e., PGPRs and symbiotic fungi, which strengthen plant defenses will generate valuable information on how pathosystems actually function in nature, and thereby provide clues to solving disease problems that engender major losses in crops every year.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.