Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology

International Journal of Molecular Sciences

Li-Chun Zhao¹  Ying He²  Xin Deng²  Xiang-Hua Xia¹  Jian Liang²  Geng-Liang Yang¹  Wei Li³ 

[1]College of Pharmacy, Hebei University, Baoding 071002, China

[2] E-Mails:

[3]The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China

[4] E-Mails:

[5]College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China

[6] E-Mail:

关键词: ultrasound-assisted extraction;    syringin;    Ilex rotunda;    response surface methodology;    Box-Behnken design;   

DOI  :  10.3390/ijms13067607

来源: mdpi

【 摘 要 】

In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE) of syringin from the bark of Ilex rotunda Thumb using response surface methodology (RSM) is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD) to obtain the highest extraction efficiency. The optimal conditions were the use of a sonication frequency of 40 kHz, 65% methanol as the solvent, an extraction time of 30 min and an extraction temperature of 40 °C. Using these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing syringin extraction from the bark of I. rotunda.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.