Restoration of Electric Footshock-Induced Immunosuppression in Mice by Gynostemma pentaphyllum Components

Molecules

Sun-A Im¹  Hyun Sook Choi¹  Soon Ok Choi¹  Ki-Hyang Kim¹  Seungjeong Lee¹  Bang Yeon Hwang¹  Myung Koo Lee¹ 

[1] College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea

关键词: Gynostemma pentaphyllum;    electric footshock;    stress;    immunomodulation;    cytotoxic T lymphocytes;   

DOI  :  10.3390/molecules17077695

来源: mdpi

【 摘 要 】

The immunomodulatory effects of the ethanol extract of Gynostemma pentaphyllum (GP-EX) were examined in electric footshock (EFS)-stressed mice. The mice were orally administered various doses of GP-EX for 7 days before exposure to EFS (duration: 3 min, interval: 10 s, intensity: 2 mA) once a day from day 8 for 14 days with continuous daily feeding of GP-EX. Oral administration of GP-EX to mice prevented EFS stress-induced immunosuppression as determined by the lymphoid organ (thymus and spleen) weight and cellularity. In addition, oral administration of GP-EX restored EFS-suppressed functional properties of mature lymphocytes in terms of concanavalin A-induced proliferation of splenocytes and lipopolysaccharide-induced cytokine production (TNF-α, IL-1β). Furthermore, we found that mice that were orally administered with GP-EX generated much more potent ovalbumin-specific cytotoxic T lymphocyte responses upon intravenous ovalbumin injection compared to the untreated controls. These results demonstrate that oral administration of the ethanol extract of Gynostemma pentaphyllum could increase host defense in immunocompromised situations such as stress-induced immunosuppression.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.