Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis

Molecules

Xuan Zhang¹  Liu-Meng Yang¹  Guang-Ming Liu¹  Ya-Juan Liu¹  Chang-Bo Zheng¹  Yong-Jun Lv¹  Hao-Zhi Li¹ 

[1] ¹Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Science & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China

关键词: Pinus yunnanensis;    pine cone;    anti-HIV activity;    reverse transcriptase;    fusion;   

DOI  :  10.3390/molecules17066916

来源: mdpi

【 摘 要 】

The anti-HIV activities of a pine cone extract (YNS-PY-F) from Pinus yunnanensis have been evaluated, and its mechanisms of action were also explored. The pine cone extract, YNS-PY-F, potently inhibited HIV-1_IIIB, HIV-1_RF, HIV-1_A17, HIV-1_AO18 and HIV-2_ROD and induced cytopathic effect in C8166 cells with EC₅₀ values of 0.96 μg/mL, 1.53 μg/mL, 0.88 μg/mL, 7.20 μg/mL and 6.17 μg/mL, respectively. The quantification of a p24 production assay showed that YNS-PY-F significantly inhibited the acute replication of HIV-1_IIIB, HIV-1_RF, HIV-1_A17 and HIV-1_AO18 in C8166 cells. An MTT assay showed that YNS-PY-F also significantly inhibited the HIV-1_IIIB induced cytolysis in MT-4 cells with an EC₅₀ value of 2.22 μg/mL. The mechanism assays showed that YNS-PY-F had potent inhibitory effects on the fusion between infected cells and uninfected cells, and the activity of HIV-1 reverse transcriptase, with EC₅₀ values of 7.60 μg/mL and 4.60 μg/mL, respectively. Overall, these data suggest that the pine cone extract from Pinus yunnanensis has potent inhibitory activities against HIV-1_IIIB, HIV-1_RF, RT inhibitor-resistant strains HIV-1_A17 and HIV-1_AO18, and HIV-2_ROD, and its anti-HIV mechanisms include inhibition of HIV entry and inhibition of reverse transcriptase activity.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.