Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)

International Journal of Molecular Sciences

Shengli Jing¹  Xi Zhou¹  Hangjin Yu¹  Bingfang Liu¹  Chunxiao Zhang¹  Shuzhen Wang¹  Xinxin Peng¹  Lili Zhu¹  Yi Ding¹ 

[1] State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China;

关键词: Nilaparvata lugens;    microsatellites;    polymorphism;    genetic diversity;   

DOI  :  10.3390/ijms13089527

来源: mdpi

【 摘 要 】

Brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.