Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

Molecules

Guilong Yan²  Lilian Ji¹  Yuming Luo¹ 

[1] Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China;College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China

关键词: Eupatorium lindleyanum DC.;    high-speed counter-current chromatography;    sesquiterpenoid lactone;    separation;   

DOI  :  10.3390/molecules17089002

来源: mdpi

【 摘 要 】

A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxy-tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and ¹H-NMR.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.