Involvement of Disperse Repetitive Sequences in Wheat/Rye Genome Adjustment

International Journal of Molecular Sciences

Diana Tomás¹  Miguel Bento¹  Wanda Viegas¹ 

[1] Centro de Botânica Aplicada à Agricultura, Secção de Genética, Instituto Superior de Agronomia, Technical University of Lisbon, Tapada da Ajuda, Lisboa 1349-017, Portugal;

关键词: genome adjustment;    triticale;    wheat-rye addition lines;    dispersed repetitive sequences;    pSc20H;   

DOI  :  10.3390/ijms13078549

来源: mdpi

【 摘 要 】

The union of different genomes in the same nucleus frequently results in hybrid genotypes with improved genome plasticity related to both genome remodeling events and changes in gene expression. Most modern cereal crops are polyploid species. Triticale, synthesized by the cross between wheat and rye, constitutes an excellent model to study polyploidization functional implications. We intend to attain a deeper knowledge of dispersed repetitive sequence involvement in parental genome reshuffle in triticale and in wheat-rye addition lines that have the entire wheat genome plus each rye chromosome pair. Through Random Amplified Polymorphic DNA (RAPD) analysis with OPH20 10-mer primer we unraveled clear alterations corresponding to the loss of specific bands from both parental genomes. Moreover, the sequential nature of those events was revealed by the increased absence of rye-origin bands in wheat-rye addition lines in comparison with triticale. Remodeled band sequencing revealed that both repetitive and coding genome domains are affected in wheat-rye hybrid genotypes. Additionally, the amplification and sequencing of pSc20H internal segments showed that the disappearance of parental bands may result from restricted sequence alterations and unraveled the involvement of wheat/rye related repetitive sequences in genome adjustment needed for hybrid plant stabilization.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.