Real Time Anti-Toxoplasma gondii Activity of an Active Fraction of Eurycoma longifolia Root Studied by in Situ Scanning and Transmission Electron Microscopy

Molecules

Nowroji Kavitha²  Rahmah Noordin²  Chan Kit-Lam¹ 

[1] School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia;Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia

关键词: Toxoplasma gondii;    Eurycoma longifolia;    transmission electron microscope;    scanning electron microscope;    toxoplasmosis;   

DOI  :  10.3390/molecules17089207

来源: mdpi

【 摘 要 】

The inhibitory effect of active fractions of Eurycomalongifolia (E. longifolia)root, namely TAF355 and TAF401, were evaluated against Toxoplasma gondii (T. gondii). In our previous study, we demonstrated that T. gondii was susceptible to TAF355 and TAF401 with IC₅₀ values of 1.125 µg/mL and 1.375 µg/mL, respectively. Transmission (TEM) and scanning electron microscopy (SEM) observations were used to study the in situ antiparasitic activity at the IC₅₀ value. Clindamycin was used as positive control. SEM examination revealed cell wall alterations with formation of invaginations followed by completely collapsed cells compared to the normal T. gondii cells in response to the fractions. The main abnormality noted via TEM study was decreased cytoplasmic volume, leaving a state of structural disorganization within the cell cytoplasm and destruction of its organelles as early as 12 h of treatment, which indicated of rapid antiparasitic activity of the E. longifolia fractions. The significant antiparasitic activity shown by the TAF355 and TAF401 active fractions of E. longifolia suggests their potential as new anti-T. gondii agent candidates.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.