International Journal of Molecular Sciences | |
Evaluation of Sex-Specific Gene Expression in Archived Dried Blood Spots (DBS) | |
James H. Resau2  Nhan T. Ho4  Karl Dykema3  Matthew S. Faber5  Julia V. Busik5  Radoslav Z. Nickolov1  Kyle A. Furge3  Nigel Paneth4  Scott Jewell2  | |
[1] Department of Mathematics & Computer Science, Fayetteville State University, Fayetteville, NC 28301, USA; E-Mail:;Program of Biospecimen Science, Van Andel Research Institute, Grand Rapids, MI 49503, USA; E-Mails:;Laboratory of Computational Biology, Van Andel Research Institute, Grand Rapids, MI 49503, USA; E-Mails:;Department of Epidemiology & Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI 48823, USA; E-Mails:;Department of Physiology, Michigan State University, East Lansing, MI 48824, USA; E-Mails: | |
关键词: archived dried blood spots (DBS); sex-specific; gene expression; molecular genetic profiling; microarray; | |
DOI : 10.3390/ijms13089599 | |
来源: mdpi | |
【 摘 要 】
Screening newborns for treatable serious conditions is mandated in all US states and many other countries. After screening, Guthrie cards with residual blood (whole spots or portions of spots) are typically stored at ambient temperature in many facilities. The potential of archived dried blood spots (DBS) for at-birth molecular studies in epidemiological and clinical research is substantial. However, it is also challenging as analytes from DBS may be degraded due to preparation and storage conditions. We previously reported an improved assay for obtaining global RNA gene expression from blood spots. Here, we evaluated sex-specific gene expression and its preservation in DBS using oligonucleotide microarray technology. We found X inactivation-specific transcript (
【 授权许可】
CC BY
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
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RO202003190042801ZK.pdf | 462KB | download |