Arsenic Trioxide Inhibits Cell Growth and Induces Apoptosis through Inactivation of Notch Signaling Pathway in Breast Cancer

International Journal of Molecular Sciences

Jun Xia²  Youjian Li⁵  Qingling Yang⁴  Chuanzhong Mei²  Zhiwen Chen²  Bin Bao³  Aamir Ahmad³  Lucio Miele¹  Fazlul H Sarkar³ 

[1] University of Mississippi Cancer Institute, 2500 N State St, Jackson, MS 39216, USA; E-Mail:;Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu 233030, China; E-Mails:;Department of Pathology and Oncology, Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA; E-Mails:;Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu 233030, China; E-Mail:;Laboratory Medicine, Taixing People’s Hospital, Taizhou 225400, China; E-Mail:

关键词: Notch;    arsenic trioxide;    NF-κB;    breast cancer;    apoptosis;    cell growth;   

DOI  :  10.3390/ijms13089627

来源: mdpi

【 摘 要 】

Arsenic trioxide has been reported to inhibit cell growth and induce apoptotic cell death in many human cancer cells including breast cancer. However, the precise molecular mechanisms underlying the anti-tumor activity of arsenic trioxide are still largely unknown. In the present study, we assessed the effects of arsenic trioxide on cell viability and apoptosis in breast cancer cells. For mechanistic studies, we used multiple cellular and molecular approaches such as MTT assay, apoptosis ELISA assay, gene transfection, RT-PCR, Western blotting, and invasion assays. For the first time, we found a significant reduction in cell viability in arsenic trioxide-treated cells in a dose-dependent manner, which was consistent with induction of apoptosis and also associated with down-regulation of Notch-1 and its target genes. Taken together, our findings provide evidence showing that the down-regulation of Notch-1 by arsenic trioxide could be an effective approach, to cause down-regulation of Bcl-2, and NF-κB, resulting in the inhibition of cell growth and invasion as well as induction of apoptosis. These results suggest that the anti-tumor activity of arsenic trioxide is in part mediated through a novel mechanism involving inactivation of Notch-1 and its target genes. We also suggest that arsenic trioxide could be further developed as a potential therapeutic agent for the treatment of breast cancer.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.