Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage

Journal of Functional Biomaterials

Mari Huttu²  Siru Turunen²  Viktoria Sokolinski²  Virpi Tiitu¹  Mikko Lammi¹ 

[1] Institute of Biomedicine, University of Eastern Finland, P.O. Box 1627, Kuopio FI-70211, Finland;Department of Applied Physics, University of Eastern Finland, P.O. Box 1627, Kuopio FI-70211, Finland;

关键词: chondrocytes;    medium;    temperature;    confocal microscopy;    osmolarity;   

DOI  :  10.3390/jfb3030544

来源: mdpi

【 摘 要 】

Osmotic loading of articular cartilage has been used to study cell-tissue interactions and mechanisms in chondrocyte volume regulation in situ. Since cell volume changes are likely to affect cell’s mechanotransduction, it is important to understand how environmental factors, such as composition of the immersion medium and temperature affect cell volume changes in situ in osmotically challenged articular cartilage. In this study, chondrocytes were imaged in situ with a confocal laser scanning microscope (CLSM) through cartilage surface before and 3 min and 120 min after a hypo-osmotic challenge. Samples were measured either in phosphate buffered saline (PBS, without glucose and Ca²⁺) or in Dulbecco’s modified Eagle’s medium (DMEM, with glucose and Ca²⁺), and at 21 °C or at 37 °C. In all groups, cell volumes increased shortly after the hypotonic challenge and then recovered back to the original volumes. At both observation time points, cell volume changes as a result of the osmotic challenge were similar in PBS and DMEM in both temperatures. Our results indicate that the initial chondrocyte swelling and volume recovery as a result of the hypo-osmotic challenge of cartilage are not dependent on commonly used immersion media or temperature.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.