Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management

Insects

Cheryl Jenkins¹  Toni A. Chapman²  Jessica L. Micallef² 

[1] Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Woodbridge Road, Menangle, NSW 2568, Australia;

关键词: Diptera;    Tephritidae;    parasitoid;    biological control;    DNA barcode;    PCR;    microsatellites;   

DOI  :  10.3390/insects3030763

来源: mdpi

【 摘 要 】

Parasitoid detection and identification is a necessary step in the development and implementation of fruit fly biological control strategies employing parasitoid augmentive release. In recent years, DNA-based methods have been used to identify natural enemies of pest species where morphological differentiation is problematic. Molecular techniques also offer a considerable advantage over traditional morphological methods of fruit fly and parasitoid discrimination as well as within-host parasitoid identification, which currently relies on dissection of immature parasitoids from the host, or lengthy and labour-intensive rearing methods. Here we review recent research focusing on the use of molecular strategies for fruit fly and parasitoid detection and differentiation and discuss the implications of these studies on fruit fly management.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.