Oxidative Response and Antioxidative Mechanism in Germinating Soybean Seeds Exposed to Cadmium

International Journal of Environmental Research and Public Health

Shiyong Yang²  Jianchun Xie¹ 

[1] School of Environmental Science and Engineering, Anhui Normal University, Wuhu 241000, China;Section of Ecology, School of Life Sciences, Anhui Normal University, Wuhu 241000, China;

关键词: cadmium;    soybean;    oxidative stress;    antioxidative response;    bioindicator;   

DOI  :  10.3390/ijerph9082827

来源: mdpi

【 摘 要 】

Seeds of soybean (Glycine max L.) exposed to 50 mg/L (Cd50), 100 mg/L (Cd100) and 200 mg/L (Cd200) cadmium solution for 24, 48, 72 and 96 h were examined with reference to Cd accumulation, oxidative stress and antioxidative responses. Soybean seeds accumulated Cd in an exposure time-and dosage-dependent manner. FRAP (ferric reducing ability of plasma) concentration, GSH/hGSH content, and GST activity showed a pronounced exposure time-dependent response. Cd100 enhanced FRAP concentration in germinating soybean seeds as compared to Cd50 treatment after 24 h exposure. Cd200 however increased statistically GST activities after 72 and 96 h exposure. Under all Cd dosages, GSH/hGSH concentrations were depressed with increasing exposure time. Reduction of GSH/hGSH content and concomitant increase of GST activity suggested a possible participation of GSH into GSH-Cd conjugates synthesis. MDA content is a potential biomarker for monitoring Cd phytotoxicity because it responds significantly to treatment dosage, exposure time and dosage × exposure time interaction. Increase of proline content may be a response to acute heavy metal toxicity in soybean seeds.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.