Physical Properties of Polypeptide Electrospun Nanofiber Cell Culture Scaffolds on a Wettable Substrate

Polymers

Donald T. Haynie¹  Dhan B. Khadka¹ 

[1] Nanomedicine and Nanobiotechnology Laboratory, Department of Physics, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA;

关键词: cell;    electrospinning;    fiber;    physics;    polypeptide;    scaffold;   

DOI  :  10.3390/polym4031535

来源: mdpi

【 摘 要 】

Physical properties of poly(L-ornithine) (PLO), a polycation, poly(L-glutamic acid₄-co-L-tyrosine) (PLEY), a polyanion, and electrospun fibers made of these polymers have been determined and compared. The polymers adopted random coil-like conformations in aqueous feedstocks at neutral pH and in dehydrated cast films and fibers on glass, and the fibers comprised numerous counterions, according to spectral analysis. Adsorption of model proteins and serum proteins onto hydrated and crosslinked fibers depended on the electrical charge of the proteins and the fibers. The surface charge density of the fibers will be comparable to, but less than, the charge density on the outer leaflet of the plasma membrane of usual eukaryotic cells. The present analysis thus advances understanding of cell behavior on electrospun fiber scaffolds, a topic of considerable current interest.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.