Antioxidant Profile of Trifolium pratense L.

Molecules

Biljana Kaurinovic¹  Mira Popovic¹  Sanja Vlaisavljevic¹  Heidy Schwartsova¹ 

[1] ¹Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia ²Middle European High School, Kráľovská 386/11, Skalica 90901, Slovakia ³Department of Environmental Protection, Faculty of Technical Sciences, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia

关键词: Trifolium pratense;    antioxidant activity;    flavonoids;    biochemical parameters;   

DOI  :  10.3390/molecules170911156

来源: mdpi

【 摘 要 】

In order to examine the antioxidant properties of five different extracts of Trifolium pratense L. (Leguminosae) leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H₂O and (to some extent) the EtOAc extracts showed a potent antioxidant effect compared with BHT and BHA, well-known synthetic antioxidants. In addition, in vivo experiments were conducted with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood of mice after their treatment with extracts of T. pratense leaves, or in combination with CCl₄. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence of the selected flavonoids: quercetin, luteolin, apigenin, naringenin and kaempferol, which were studied using a HPLC-DAD technique. HPLC-DAD analysis showed a noticeable content of natural products according to which the examined Trifolium pratense species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.