Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves

Molecules

Ramiro Quintanilla-Licea¹  Rolando Morado-Castillo¹  Ricardo Gomez-Flores³  Hartmut Laatsch²  Mar໚ Julia Verde-Star¹  Humberto Hernández-Martínez³  Patricia Tamez-Guerra³  Reyes Tamez-Guerra³ 

[1] Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza 66451, Nuevo León, Mexico;Institute of Organic and Biomolecular Chemistry, Georg-August-University of Göttingen, Tammannstrasse 2, Göttingen D-37077, Germany;Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza 66451, Nuevo León, Mexico

关键词: Gymnosperma glutinosum;    asteraceae;    Mexican medicinal plant;    lymphoma cells;    cancer;    ent-Labdanediterpenes;   

DOI  :  10.3390/molecules170911229

来源: mdpi

【 摘 要 】

Bioassay-guided fractionation of hexane extracts of Gymnosperma glutinosum (Asteraceae) leaves, collected in North Mexico, afforded the known compounds hentriacontane (1) and (+)-13S,14R,15-trihydroxy-ent-labd-7-ene (2), as well as the new ent-labdane diterpene (−)-13S,14R,15-trihydroxy-7-oxo-ent-labd-8(9)-ene (3). In addition, D-glycero-D-galactoheptitol (4) was isolated from the methanolic extract of this plant. Their structures were established on the basis of high-field 1D- and 2D NMR methods supported by HR-MS data. The cytotoxic activity was determined by using the in vitro L5178Y-R lymphoma murine model. Hentriacontane (1) and the new ent-labdane 3 showed weak cytotoxicity, whereas the ent-labdane 2 showed significant (p < 0.05) and concentration dependent cytotoxicity (up to 78%) against L5178Y-R cells at concentrations ranging from 7.8 to 250 µg/mL.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.