PepGMV Rep-Protein Expression in Mammalian Cells

Viruses

Angela Mar໚ Chapa-Oliver¹  Laura Mej໚-Teniente¹  Teresa Garc໚-Gasca²  Ramon Gerardo Guevara-Gonzalez¹ 

[1] CA Ingeniería de Biosistemas, División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Santiago de Querétaro, Qro., 76010, México;División de Investigación y Posgrado, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias s/n, Juriquilla, Qro., 76230, México;

关键词: Geminivirus;    cell cycle;    Rep protein;   

DOI  :  10.3390/v4091792

来源: mdpi

【 摘 要 】

The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.