Using Complementary Acoustic and Optical Techniques for Quantitative Monitoring of Biomolecular Adsorption at Interfaces

Biosensors

Rupert Konradi¹  Marcus Textor² 

[1] BASF SE, Advanced Materials and Systems Research, D-67056, Ludwigshafen, Germany; E-Mail:;Laboratory for Surface Science and Technology, Department of Materials, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland; E-Mail:

关键词: quartz crystal microbalance with dissipation monitoring (QCM-D);    surface plasmon resonance (SPR);    optical waveguide lightmode spectroscopy (OWLS);    dual polarization interferometry (DPI);    hydration;    polymer brush;    supported lipid bilayer (SLB);    protein adsorption kinetics;    conformational changes;    PMOXA;   

DOI  :  10.3390/bios2040341

来源: mdpi

【 摘 要 】

The great wealth of different surface sensitive techniques used in biosensing, most of which claim to measure adsorbed mass, can at first glance look unnecessary. However, with each technique relying on a different transducer principle there is something to be gained from a comparison. In this tutorial review, different optical and acoustic evanescent techniques are used to illustrate how an understanding of the transducer principle of each technique can be exploited for further interpretation of hydrated and extended polymer and biological films. Some of the most commonly used surface sensitive biosensor techniques (quartz crystal microbalance, optical waveguide spectroscopy and surface plasmon resonance) are briefly described and five case studies are presented to illustrate how different biosensing techniques can and often should be combined. The case studies deal with representative examples of adsorption of protein films, polymer brushes and lipid membranes, and describe e.g., how to deal with strongly vs. weakly hydrated films, large conformational changes and ordered layers of biomolecules. The presented systems and methods are compared to other representative examples from the increasing literature on the subject.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.