A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles

Sensors

Suyun Zeng¹  Sujun Wang²  Li Wang¹  Lihua Yang¹  Zhenbo Chen¹ 

[1] Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China; E-Mails:;Department of Biomedical engineering, Chongqing University, 174 Shazhengjie, Shapingba District, Chongqing 400044, China; E-Mail:

关键词: CD105;    immunosensor;    nanomaterial;   

DOI  :  10.3390/s121013471

来源: mdpi

【 摘 要 】

CD105 is a well-known tumor metastasis marker and useful for early monitoring of metastasis and cancer relapse. It is important to generate rapid, reliable and precise analytical information regarding CD105 levels. To establish a simple, selective and sensitive detection method, we prepared an immunosensor with novel bioconjugates based on Pt nanoparticles, thionin acetate and antibodies. The proposed immunosensor displayed a broader linear response to CD105, with a working range of 1.3 to 200.0 ng/mL and a detection limit of 0.9 ng/mL under optimal conditions. Moreover, the studied immunosensor exhibited high sensitivity, fast analysis and adequate stability. The proposed methodology could readily be extended to other clinical- or environment-related biospecies.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.