Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage

Biosensors

Minhui Dai¹  Shengquan Jin¹ 

[1] University of Massachusetts, 102 Holdsworth Way, Amherst, MA 01003, USA; E-Mails:

关键词: polyvinylpyrrolidone (PVP);    nanofiber;    electrospinning;    microfluidic biosensor;    enzyme storage;   

DOI  :  10.3390/bios2040388

来源: mdpi

【 摘 要 】

This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155 ± 34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the change in absorption over time. Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%. After a storage study over 280 days, 40% of the activity remained. In addition to activity, the fibers were observed to solubilize in the microfluidic chamber. The chromogenic 3,3′,5,5′-tetramethylbenzidine solution reacted immediately with the fibers as they passed through a microfluidic channel. The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.