Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein

Antibodies

Yukie Murakami-Yamaguchi⁵  Junko Hirose⁴  Kumiko Kizu²  Fumiko Okazaki¹  Wataru Fujii³ 

[1] Department of Food and Nutrition, Kyoto Women’s University, Kitahiyoshi-cho 35, Imakumano, Higashiyama-ku, Kyoto 605-8501, Japan; E-Mail:;Department of Life and Living, Osaka Seikei College, Aikawa 3-10-62, Higashiyodogawa, Osaka 533-0007, Japan; E-Mail:;Suntory Business Expert Limited, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan; E-Mail:;Department of Food and Nutrition, School of Human Cultures, The University of Shiga Prefecture, Hassaka-cho 2500, Hikone, Shiga 522-8533, Japan; E-Mail:;Kyoto College of Nutritional & Medical, Setogawa-cho 18, Saga Tenryuji, Ukyo-ku, Kyoto 616-8376, Japan; E-Mail:

关键词: lipid transfer protein;    beer;    ELISA;    food allergy;    monoclonal antibody;   

DOI  :  10.3390/antib1030259

来源: mdpi

【 摘 要 】

Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL and no cross-reactivity with wheat, adlay and rye. Using this ELISA, LTP was determined in several types of barley-foods, including fermented foods such as malt vinegar, barley-malt miso and beer. LTP content in beer of the same kind was approximately constant, even if manufacturing factory and production days were different. Not only as a factor of foam formation and stability but also as an allergen, controlling and monitoring of LTP in beer should be considered. Taken together, our LTP-detecting ELISA can be proposed as an appropriate system for the quality control of beer.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.