Ammonificins C and D, Hydroxyethylamine Chromene Derivatives from a Cultured Marine Hydrothermal Vent Bacterium, Thermovibrio ammonificans

Marine Drugs

Eric H. Andrianasolo²  Liti Haramaty²  Richard Rosario-Passapera²  Costantino Vetriani²  Paul Falkowski²  Eileen White¹ 

[1]Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, 679 Hoes Lane, Piscataway, NJ 08854, USA

[2]Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA

关键词: marine natural product;    deep-sea hydrothermal vent;    drug discovery;    induction of apoptosis;    bacteria;    computational methods;   

DOI  :  10.3390/md10102300

来源: mdpi

【 摘 要 】

Chemical and biological investigation of the cultured marine hydrothermal vent bacterium, Thermovibrio ammonifican led to the isolation of two hydroxyethylamine chromene derivatives, ammonificins C and D. Their structures were elucidated using combination of NMR and mass spectrometry. Absolute stereochemistry was ascertained by comparison of experimental and calculated CD spectra. Biological evaluation and assessment were determined using the patented ApopScreen cell-based screen for apoptosis-induction. Ammonificins C and D induce apoptosis in micromolar concentrations. To our knowledge, this finding is the first report of chemical compounds that induce apoptosis from the cultured deep-sea marine organism, hydrothermal vent bacterium, Thermovibrio ammonificans.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.