Multiple Strategies for Translesion Synthesis in Bacteria

Cells

Paul J. Ippoliti²  Nicholas A. DeLateur²  Kathryn M. Jones¹ 

[1]Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA

[2]Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA

关键词: DNA damage;    mutagenesis;    SOS response;    DNA pol IV (DinB);    2C)"'>DNA pol V (UmuD'₂C);    dnaE;    dnaE2;    imuA;    imuB;    imuC;   

DOI  :  10.3390/cells1040799

来源: mdpi

【 摘 要 】

Damage to DNA is common and can arise from numerous environmental and endogenous sources. In response to ubiquitous DNA damage, Y-family DNA polymerases are induced by the SOS response and are capable of bypassing DNA lesions. In Escherichia coli, these Y-family polymerases are DinB and UmuC, whose activities are modulated by their interaction with the polymerase manager protein UmuD. Many, but not all, bacteria utilize DinB and UmuC homologs. Recently, a C-family polymerase named ImuC, which is similar in primary structure to the replicative DNA polymerase DnaE, was found to be able to copy damaged DNA and either carry out or suppress mutagenesis. ImuC is often found with proteins ImuA and ImuB, the latter of which is similar to Y‑family polymerases, but seems to lack the catalytic residues necessary for polymerase activity. This imuAimuBimuC mutagenesis cassette represents a widespread alternative strategy for translesion synthesis and mutagenesis in bacteria. Bacterial Y‑family and ImuC DNA polymerases contribute to replication past DNA damage and the acquisition of antibiotic resistance.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.