Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

International Journal of Molecular Sciences

Jyh-Ming Wu¹  Shin-Yao Wang² 

[1] Department of Chemical and Materials Engineering, Chinese Culture University, 55, Hwa-Kang Rd., Yang-Ming-Shan, Taipei 111-14, Taiwan; E-Mail:;Institute of Nanomaterials, Chinese Culture University, 55, Hwa-Kang Rd., Yang-Ming-Shan, Taipei 111-14, Taiwan; E-Mail:

关键词: Vitreoscilla hemoglobin;    lower temperature;    Pichia pastoris;    AOX 1 promoter;    recombinant protein production;   

DOI  :  10.3390/ijms131013212

来源: mdpi

【 摘 要 】

An heterologous expression of Vitreoscilla hemoglobin (VHb) for improving cell growth and recombinant protein production has been successfully demonstrated in various hosts, including Pichia pastoris. Lower temperature cultures can enhance target protein production in some studies of P. pastoris. In this study, the strategy of combining heterologous VHb expression and lower temperature cultures in P. pastoris showed that final cell density and viability of VHb⁺ strain at 23 °C were higher than that at 30 °C. In addition, the effects of VHb expression on recombinant β-galactosidase production and oxygen uptake rate were also higher at 23 °C than at 30 °C. Consequently, lower temperature cultures can enlarge VHb effectiveness on cell performance of P. pastoris. This is because VHb activity obtained at 23 °C cultures was twofold higher than that at 30 °C cultures, due to a different heme production. This strategy makes P. pastoris an excellent expression host particularly suitable for increasing the yields of the low-stability and aggregation-prone recombinant proteins.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.