Comparative Antitumor Effect of Preventive versus Therapeutic Vaccines Employing B16 Melanoma Cells Genetically Modified to Express GM-CSF and B7.2 in a Murine Model

Toxins

Antonio Miguel²  Mar໚ José Herrero²  Luis Sendra²  Rafael Botella³  Rosa Algás¹  Maria Sánchez² 

[1] Radiotherapy, Hospital Clínico Universitario, Av. Blasco Ibáñez, 10, Valencia 46010, Spain;Department of Pharmacology, Faculty of Medicine, University of Valencia, Avda. Blasco Ibáñez, 15, Valencia 46010, Spain;Dermatology, Hospital Universitario y Politécnico La Fe, Bulevar Sur s/n, Valencia 46026, Spain;

关键词: cancer vaccines;    gene therapy;    non-viral;    GM-CSF;    B7.2;   

DOI  :  10.3390/toxins4111058

来源: mdpi

【 摘 要 】

Cancer vaccines have always been a subject of gene therapy research. One of the most successful approaches has been working with genetically modified tumor cells. In this study, we describe our approach to achieving an immune response against a murine melanoma model, employing B16 tumor cells expressing GM-CSF and B7.2. Wild B16 cells were injected in C57BL6 mice to cause the tumor. Irradiated B16 cells transfected with GM-CSF, B7.2, or both, were processed as a preventive and therapeutic vaccination. Tumor volumes were measured and survival curves were obtained. Blood samples were taken from mice, and IgGs of each treatment group were also measured. The regulatory T cells (Treg) of selected groups were quantified using counts of images taken by confocal microscopy. Results: one hundred percent survival was achieved by preventive vaccination with the group of cells transfected with p2F_GM-CSF. Therapeutic vaccination achieved initial inhibition of tumor growth but did not secure overall survival of the animals. Classical Treg cells did not vary among the different groups in this therapeutic vaccination model.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.