A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae

Energies

Wei Han¹  Chunying Li¹  Xiaoling Miao¹ 

[1] School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; E-Mails:

关键词: microalgae;    miniature;    screening;    CO₂ fixation;    wastewater;   

DOI  :  10.3390/en5114372

来源: mdpi

【 摘 要 】

In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO₂ fixation. Cell growth under different CO₂ supply conditions (0.2, 0.4, 0.8, and 1.2 g L⁻¹ d⁻¹), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species (Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum, Scenedesmus obliquus SJTU-3, Chlamydomonas sajao, Dunaliella primolecta) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437g L⁻¹) in anaerobic pond water with 0.8, and 1.2 g L⁻¹ d⁻¹ CO₂. The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.