Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles

Molecules

Li Cui¹  E Sun¹  Zhen-Hai Zhang¹  Xiao-Bin Tan¹  Ying-Jie Wei¹  Xin Jin¹ 

[1] Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, Jiangsu, China;

关键词: Epimedium;    suet oil;    self-assembled nanomicelles;    icariin;    Caco-2 cell monolayers;    single-pass rat intestinal perfusion;   

DOI  :  10.3390/molecules171112984

来源: mdpi

【 摘 要 】

The purpose of this work was to research the enhancement of Epimedium fried with suet oil based on the in vivo formation self-assembled flavonoid nanomicelles. Taking icariin as the representative, under the action of suet oil, self-assembled nanomicelles were prepared under simulated gastrointestinal tract conditions and were characterized by dynamic light scattering and transmission electron microscopy (TEM). The experiments with icariin self-assembled nanomicelles without suet oil were done according to the above. The influence of suet oil on the transportation of icariin across Caco-2 cell monolayers and the absorption in rat intestine of self-assembled nanomicelles were evaluated. The particle size of icariin self-assembled nanomicelles with suet oil was smaller than without suet oil. The nanomicelles seemed to be monodisperse spherical particle with smooth surfaces. The icariin entrapment efficiency of self-assembled nanomicelles with suet oil was increased from 43.1% to 89.7%. In Caco-2 cell monolayers, the absorptive permeability, secretory permeability and efflux ratio of icariin self-assembled nanomicelles with suet oil was 1.26 × 10⁻⁶ cm/s, 5.91 × 10⁻⁶ cm/s and 4.69, respectively, while that of icariin self-assembled nanomicelles without suet oil was 0.62 × 10^{−6 cm/s}, 3.00 × 10⁻⁶ cm/s, and 4.84, respectively. In rat intestinal perfusion experiments, the permeability coefficient of icariin self-assembled nanomicelles with suet oil in duodenum was higher than the value of icariin self-assembled nanomicelles without suet oil (p < 0.05). With the action of suet oil, icariin self-assembled nanomicelles were more stable and the entrapment efficiency was higher than that without suet oil, which could increase the solubility of icariin and improve its intestinal absorption. Therefore, suet oil plays a role in its enhancement.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.