Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate

Antibodies

Hiroyuki Tanaka²  Madan K. Paudel²  Ayako Takei²  Junichi Sakoda²  Thaweesak Juengwatanatrakul⁶  Kaori Sasaki-Tabata²  Waraporn Putalun⁴  Wanchai De-Eknamkul³  Oraphan Matangkasombut¹  Yukihiro Shoyama⁵ 

[1] Faculty ofPharmacy, Rangsit University, Pathumthani, Thailand;Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan;Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand;Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand;Faculty of Pharmaceutical Sciences, Nagasaki International University, Huis Ten Bosch 2825-7, Sasebo, Nagasaki 859-3298, Japan;Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

关键词: artemisinin;    artesunate;    Artemisia annua;    monoclonal antibody;    recombinant Fab;    ELISA;   

DOI  :  10.3390/antib1030273

来源: mdpi

【 摘 要 】

We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.